Coding

Part:BBa_K3190302:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-14)


TPST2: Tyrosylprotein sulfotransferase 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 882
    Illegal SpeI site found at 1108
    Illegal PstI site found at 32
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1108
    Illegal PstI site found at 32
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 232
    Illegal BglII site found at 804
    Illegal BamHI site found at 474
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 882
    Illegal SpeI site found at 1108
    Illegal PstI site found at 32
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 882
    Illegal SpeI site found at 1108
    Illegal PstI site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 116


Design Notes

TPST 1 and 2 are the enzymes, that facilitate tyrosine sulfation, a post translational modification essential for the function of the human LHCGR receptor. Yeast do not have these necessary enzymes (TPST1 and TPST2) by itself, which is why we use it in our construct.


Source

Homo sapiens

References